Abcam, ab114857, Anti-GW182 antibody

Size: 100µg
Rabbit Polyclonal GW182 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TNRC6A aa 1200-1300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TNRC6A aa 1200-1300. The exact immunogen used to generate this antibody is proprietary information.Q8NDV7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GW182 also known as TNRC6A serves a mechanical role as an important component in the microRNA-induced silencing complex (miRISC). This protein weighs about 182 kilodaltons and provides a platform for microRNA function. It is expressed in the cytoplasm of many cell types. GW182 interacts with Argonaute proteins which then assists in gene silencing by recruiting these proteins to mRNA targets.
Biological function summary
GW182 is involved in RNA-mediated post-transcriptional gene silencing. As part of the miRISC complex GW182 facilitates the repression or degradation of target mRNAs. This protein assists in binding with components such as PABP (poly(A)-binding protein) to regulate gene expression. This role influences cellular processes like development differentiation and response to stress.
Pathways
The actions of GW182 are linked to the RNA interference pathway and the microRNA-mediated gene silencing pathway. In these pathways GW182 collaborates closely with proteins like Argonaute (AGO) to execute its gene-silencing responsibilities. By addressing specific mRNA targets these interactions enable precise control of gene expression.
Irregularities in GW182 function connect to diseases involving dysregulated microRNA activity such as certain cancers and neurological disorders. Cancer involves aberrant expression of miRNAs which affects GW182's regulatory capacity. Another protein Dicer works alongside GW182 in generating functional miRNAs relevant in cancer pathways indicating how essential balance in this network maintains cellular health.