Abcam, ab115321, Anti-pan PDK antibody [3H5BE12]

Size: 100µg
Mouse Monoclonal PDK1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse, Recombinant full length protein - Human samples. Cited in 3 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3H5BE12,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Purification notes-Near homogeneity as judged by SDS-PAGE (>95% purity). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pan PDK or pyruvate dehydrogenase kinases are a family of enzymes that regulate glucose metabolism by inhibiting the pyruvate dehydrogenase complex (PDC). The four human PDK isoforms are known: PDK1 PDK2 PDK3 and PDK4. These kinases typically express in tissues like heart liver muscle and kidney where they play important roles in energy metabolism. The molecular weights of PDKs range from around 40 to 49 kDa.
Biological function summary
Pyruvate dehydrogenase kinases control the flux between glycolysis and the tricarboxylic acid cycle. By phosphorylating the E1α subunit of PDC PDKs decrease PDC activity slowing down the conversion of pyruvate into acetyl-CoA. In this way PDKs help balance energy production especially in tissues requiring flexible metabolic responses. This function connects them to energy homeostasis especially during starvation and exercise where fatty acids serve as the primary energy source.
Pathways
PDKs are central in key metabolic pathways like glycolysis and the tricarboxylic acid cycle. These pathways ensure energy production efficiency based on cellular needs. Through their action PDKs interact with proteins like the E2 and E3 components of PDC modifying their activities according to cellular conditions. The regulation of PDKs also involves signaling pathways affecting glucose and fat metabolism which makes them integral to broader metabolic control mechanisms.
Imbalances in PDK activity link to metabolic syndromes such as diabetes and cancer. In diabetes altered PDK expression can contribute to impaired glucose utilization impacting insulin sensitivity. In some cancers upregulated PDKs promote aerobic glycolysis known as the Warburg effect supporting rapid tumor growth. Proteins involved in insulin signaling pathways and oncogenes are often connected to PDKs in these contexts highlighting their role in disease progression and potential as therapeutic targets.