Abcam, ab116150, Recombinant human TGF beta 1 protein (Active)

Size: 5µg / 20µg / 100µg
Recombinant human TGF beta 1 protein (Active) is a Human Full Length protein, in the 279 to 390 aa range, expressed in CHO cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:CHO cells,
Tags:Tag free,
Applications:HPLC, SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Method of determination: Inhibition of IL-4-induced HT-2 cell proliferationAcceptance criteria: ED50 ≤500 pg/mL (≥ 2.0 x 10^6 units/mg)The activity of Human TFG-beta is 2.2 x 10^4 RU/μg, which was calibrated against recombinant human TGF-beta 1.,
Accession:P01137,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute at 0.1 mg/mL in 10 mM HCl,
Storage buffer:Constituents: 95% Trehalose, 0.1% Trifluoroacetic acid

Product details:
Endotoxin levels determined by LAL method.
For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)