Abcam, ab116764, Recombinant Human PPT1/PPT protein

Size: 10µg
Recombinant Human PPT1/PPT protein is a Human Full Length protein, in the 1 to 306 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:Tag free,
Applications:ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P50897,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.3% Glutathione

Product details:
This product was previously labelled as PPT1.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPT1 also known as palmitoyl-protein thioesterase 1 is an enzyme that plays a critical role in protein metabolism by removing thioester-linked palmitate groups from proteins. This enzyme has a molecular weight of approximately 37 kDa. PPT1 is expressed widely in the body with significant expression in tissues such as the brain and liver. It localizes primarily in the lysosomes where it assists in lysosomal degradation pathways. Polyclonal antibodies against PPT1 can be utilized in protein analysis and help elucidate the structure and functionality of this enzyme further.
Biological function summary
PPT1 contributes to the turnover and stability of proteins by mediating their de-palmitoylation an important step in maintaining appropriate protein function and cellular homeostasis. This enzyme assists in the recycling and degradation process by facilitating the catabolism of proteins through the lysosomal pathway. PPT1 does not function as part of a larger complex but operates individually to execute the removal of palmitate which impacts a diverse range of substrates vital for controlling cellular mechanisms such as synaptic function.
Pathways
PPT1 is heavily involved in the protein degradation pathway within lysosomes. This pathway is important for the autophagic degradation process which directly associates with cellular waste management and the recycling of cellular components. PPT1's function is also connected to protein stability because improper palmitoylation can disrupt protein location and function. The enzyme shows functional interaction with proteins like CLN2 which also participates in lysosomal degradation processes and they share involvement in neuronal homeostasis.
Defects in PPT1 activity lead to neurodegenerative conditions notably the Infantile Neuronal Ceroid Lipofuscinosis (INCL). This disorder results from the accumulation of lipid-protein complexes in neurons emphasizing the need for proper PPT1 function. PPT1 has connections to the disease context through its interaction with the protein CLN3 another gene implicated in Batten disease part of the neuronal ceroid lipofuscinoses group. The study of PPT1 opens avenues for therapeutic approaches targeting these lysosomal storage disorders. Immunohistochemistry and immunocytochemistry are techniques often used to study the role of PPT1 in these pathological states.