Abcam, ab117131, Hydroxymethylated DNA Quantification Kit (Fluorometric)

Size: 48Test / 96Test
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).
Key facts
Detection method:Fluorescent,
Sample types:Saliva, Urine, Plasma, Tissue, Suspension cells, Adherent cells,
Assay type:Quantitative,
Sensitivity:>= 10 pg/well,
Assay time:3h 20m,
Assay Platform:Microplate reader

Product details:
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in
5-methylcytosine (5-mC)
. In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide,
5-hydroxymethylcytosine (5-hmC)
, has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.
Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.
It can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric) (
ab117129
), which specifically quantifies
, for simultaneously quantification of both methylated and hydroxymethylated DNA.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols