Abcam, ab117440, Anti-DDA1 antibody

Size: 100µg
Rabbit Polyclonal DDA1 antibody. Suitable for ICC, WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DDA1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WB, ICC, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DDA1. The exact immunogen used to generate this antibody is proprietary information.Q9BW61

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DDA1 (DET1 and DDB1 associated 1) protein also known as DET1 and DDB1-associated protein 1 plays an important mechanical role in cellular processes. This protein has a molecular mass of approximately 12 kDa and is ubiquitously expressed in various tissues. DDA1 is involved in the ubiquitin-proteasome pathway acting primarily as an adaptor protein that facilitates the recruitment of substrates for ubiquitination. Its mechanical function contributes to the regulation of protein stability and degradation.
Biological function summary
DDA1 interacts with several other proteins to form part of the Cullin-RING E3 ubiquitin ligase (CRL) complex. This complex is important for tagging target proteins with ubiquitin marking them for degradation by the proteasome. DDA1's interaction with DET1 and DDB1 highlights its role in influencing cellular homeostasis and response to DNA damage. By participating in these interactions it helps maintain normal cell cycle progression and cellular response mechanisms.
Pathways
DDA1 contributes to both ubiquitin-mediated proteolysis and cell cycle regulation. Within its functioning pathways it interacts importantly with the COP9 signalosome and SCF (Skp Cullin F-box containing complex) pathway indicating its role in protein turnover and degradation. Proteins such as CUL4 and RBX1 often function in connection with DDA1 emphasizing their cooperative role in these pathways. DDA1 ensures that the cells efficiently manage their protein content affecting various cellular processes.
DDA1 presents relevance to cancer and neurodegenerative diseases. The deregulation of DDA1 expression or function can lead to impaired protein degradation contributing to the accumulation of damaged or misfolded proteins. In cancer such dysregulation may result in unchecked cell proliferation. The relationship of DDA1 with DDB1 and other CRL components highlights its importance in maintaining protein homeostasis which when disrupted can influence the progression of these diseases.