Abcam, ab119216, Bradford Reagent

Size: 500mL
Bradford Reagent (ab119216) is a quick, ready-to-use colorimetric method for total protein quantification. - Improved protein dye formulation for protein quantification, offering higher tolerance to detergent contamination - Reduced protein-to-protein variation compared to traditional Bradford assays. - Proven performance: cited in over 40 publications
Key facts
Form:LiquidSee storage information,
Storage buffer:Constituents: 14% Phosphoric acid, 3% Ethanol, 2.3% α-Cyclodextrin hydrate, 0.23% gamma-Cyclodextrin, 0.005% Brilliant Blue G

Product details:
This product is manufactured by Expedeon, an Abcam company, and was previously called Bradford Ultra - 500 ml. BFU05L is the same as the 500 ml size.
Bradford Reagent ab119216 offers an improved formulation over classical Bradford: it has higher tolerance of detergent contamination of the protein samples.
This Bradford Reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.
When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to the absorbance obtained for a series of standard protein dilutions, which are assayed alongside samples with unknown protein concentrations.
Manufactured under patent numbers: US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443.
Support on how to determine protein concentration can be found

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C