Abcam, ab119424, Anti-RGS16 antibody [OTI4E5]

Size: 100µL
Mouse Monoclonal RGS16 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse, African green monkey, Dog, Rat, Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RGS16.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OTI4E5,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human, African green monkey,
Applications:WB, IHC-P, Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human RGS16.O15492

Product details:
Clone OTI4E5 (formerly 4E5).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from cell culture supernatant by affinity chromatography, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RGS16 also known as Regulator of G-protein Signaling 16 acts as a GTPase-activating protein (GAP) specifically for Gα subunits of heterotrimeric G-proteins. By accelerating the hydrolysis of GTP to GDP RGS16 effectively turns off G-protein signaling. It has a molecular mass of approximately 23 kDa. RGS16 expresses in various tissues including the liver pancreas and brain suggesting a regulatory role in diverse physiological contexts.
Biological function summary
The G-protein signaling regulation by RGS16 affects cellular responses to extracellular signals. RGS16 is not part of a larger protein complex but interacts directly with Gα subunits to modulate their activity. Its regulatory actions facilitate precise control over signal duration contributing to processes such as cell proliferation differentiation and metabolism. These functions emphasize its importance in cellular communication and response.
Pathways
The signaling regulation by RGS16 influences the MAPK/ERK and PI3K/AKT pathways both of which are critical for regulating cell growth and survival. RGS16 interacts with G-proteins linked to receptors like GPCRs which are upstream modulators of these pathways. By influencing these pathways RGS16 therefore plays a role in controlling cell proliferation and differentiation. Another protein related to these pathways through RGS16 is Gαi which RGS16 specifically acts upon to terminate signaling.
Alterations in RGS16 expression or function can contribute to cancer and metabolic disorders such as diabetes. RGS16 influences these conditions by modulating signaling pathways that regulate cell growth and insulin signaling. In cancer RGS16 can affect tumor progression and metastasis through its interaction with the MAPK/ERK pathway. The protein also connects with insulin receptor signaling in metabolic disorders linking RGS16 to the regulation of glucose homeostasis and insulin sensitivity.