Abcam, ab120308, PP2, Src and RIP2 kinase inhibitor

Size: 1mg / 5mg / 10mg
MW 301.77 Da, Purity >98%. Inhibitor of the Src family of kinases. Inhibits Src and Lck kinase (IC 50 values are 36 and 31 nM, respectively). Also shown to inhibit RIP2 and CK1δ (IC 50 values are 19 and 41 nM, respectively).
Key facts
CAS number:172889-27-9,
Purity:>98%,
Form:SolidSee storage information,
Molecular weight:301.77 Da,
Molecular formula:C15H16ClN5,
PubChem:4878,
Nature:Synthetic,
Solubility:Soluble in DMSO to 50 mMSoluble in ethanol to 10 mM,
Biochemical name:1-tert-butyl-3-(4-chlorophenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine,
Biological description:Inhibitor of the Src family of kinases. Inhibits Src and Lck kinase (IC50 values are 36 and 31 nM, respectively). Also shown to inhibit RIP2 and CK1δ (IC50 values are 19 and 41 nM, respectively).,
Canonical smiles:CC(C)(C)N1C2=NC=NC(=C2C(=N1)C3=CC=C(C=C3)Cl)N,
InChi:InChI=1S/C15H16ClN5/c1-15(2,3)21-14-11(13(17)18-8-19-14)12(20-21)9-4-6-10(16)7-5-9/h4-8H,1-3H3,(H2,17,18,19),
InChiKey:PBBRWFOVCUAONR-UHFFFAOYSA-N,
IUPAC Name:1-tert-butyl-3-(4-chlorophenyl)pyrazolo[3,4-d]pyrimidin-4-amine

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Store under desiccating conditions, The product can be stored for up to 12 months

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Src RIP2 and Lck are important components of the non-receptor tyrosine kinase family. Src also known as proto-oncogene tyrosine-protein kinase has a mass of approximately 60 kDa. It is widely expressed in many tissue types including epithelial and immune cells. RIP2 receptor-interacting serine/threonine-protein kinase 2 has a mass of about 61 kDa and is mainly present in immune system cells. Lck lymphocyte-specific protein tyrosine kinase weighs around 56 kDa and is predominantly in T cells. These kinases phosphorylate tyrosines on substrates modulating downstream signaling pathways and influence cell growth differentiation and survival.
Biological function summary
Src RIP2 and Lck contribute to signal transduction processes critical for cellular functions. Src commonly integrates into multi-protein complexes modulating pathways linked to cell cycle progression and proliferation. RIP2 is essential for activating nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways important in inflammation and immune responses. Lck plays a pivotal role in T cell receptor (TCR) signaling initiating adaptive immune responses. These kinases often work together with other signaling proteins to ensure proper cellular outcomes in health and disease.
Pathways
Src RIP2 and Lck serve key roles in the regulation of two significant biological pathways: the NF-κB pathway and TCR signaling. Src kinase's activity modulates the activation of downstream proteins like STAT3 and FAK which influence cell adhesion and migration. RIP2 interacts with proteins such as NOD2 during innate immune responses facilitating the activation of NF-κB pathway. Lck functions upstream in TCR signaling where it is essential for phosphorylating ZAP-70 a step important for triggering downstream signaling cascades critical for T cell activation.
Dysregulation of Src RIP2 and Lck links to various pathological states. Src is notably implicated in cancer cell proliferation and metastasis making it a target in cancer therapies with PP2 Src inhibitors showing potential in treatment. RIP2 pathways are associated with inflammatory diseases like Crohn's disease as NOD2 mutations affect its interaction influencing NF-κB activation. Lck-related anomalies can contribute to immune disorders as improper TCR signaling can result in autoimmune conditions. Understanding these kinase interactions with proteins in disorders could lead to new therapeutic avenues.