Abcam, ab213776, Human ESM1 ELISA Kit (Endocan)

Size: 1 x 96Tests
Human ESM1 ELISA Kit (Endocan) is a Sandwich (quantitative) ELISA for the measurement of Human ESM1 (Endocan) in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:31.2 - 2000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human ESM1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (Endocan) (ab213776) is designed for the quantitative measurement of Human ESM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polycloncal antibody from goat specific for ESM1/Endocan has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: W20-R184) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ESM1/Endocan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ESM1/Endocan amount of sample captured in plate.
Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increased in the presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Endothelial cell-specific molecule 1 (ESM1) also known as endocan is a proteoglycan with a mass of approximately 50 kDa. It is primarily expressed in endothelial cells of blood vessels particularly in the lungs and the kidneys. ESM1 consists of a core protein covalently attached to dermatan sulfate chains. Its expression often increases under inflammatory conditions which highlights its role in vascular biology.
Biological function summary
ESM1 acts in the regulation of cell adhesion and migration processes. It interacts with leukocytes and participates in modulating inflammatory responses. ESM1 does not form part of a multi-protein complex but its activity can influence the behavior of other cell surface molecules and receptors. This function positions ESM1 as a critical player in the dynamics of vascular endothelium.
Pathways
ESM1 modulates inflammation and angiogenesis by participating in pathways involving vascular endothelial growth factor (VEGF). It interacts with proteins such as VEGF receptor-2 impacting endothelial permeability and new blood vessel formation. These interactions situate ESM1 within the signaling networks that regulate endothelial function and vascular homeostasis.
Research associates ESM1 with conditions such as sepsis and cancer. High levels of this molecule correlate with poor prognosis in these diseases linking it functionally to VEGF and other factors involved in abnormal endothelial cell activity. Understanding ESM1's role provides insights into mechanisms of disease progression and potential therapeutic targets.