Abcam, ab213791, Human IL-12 p70 ELISA Kit

Size: 1 x 96Tests
Human IL-12 p70 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-12 p70 in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 2 pg/mL,
Range:7.8 - 500 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human IL-12(p70) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213791) is designed for the quantitative detection of Human IL-12(p70) in cell culture supernatants and serum.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-12(p70) has been pre-coated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: (I23-S328)+(R23-S219)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(p70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-12(p70) amount of sample captured in plate. The standard product used in this kit is recombinant human IL-12(p70) with the molecular mass of 75 kDa.
Interleukin (IL)-12 is a 70 kDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. The IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT-18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12(p70).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C