Abcam, ab215090, Mouse SAA ELISA Kit

Size: 1 x 96Tests
Mouse SAA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse SAA with a sensitivity of 39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 39 pg/mL,
Range:0.22 - 2.5 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse SAA ELISA Kit ab215090 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse SAA in citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 39 pg/mL.
How the assay works
Mouse SAA SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse SAA ELISA Kit ab215090 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Serum Amyloid A antibody [EPR19235-167] - BSA and Azide free (Capture)
ab242783
- Anti-Serum Amyloid A antibody [EPR19235-149] - BSA and Azide free (Detector)
ab242783
Mouse SAA1 (serum amyloid A-1 protein) is a secreted 102 amino acid, acute phase protein encoded by the Saa1 gene. The SAA proteins are a family of apolipoproteins that are associated with high-density lipoprotein (HDL). Four SAA isoforms have been currently characterized in mice; SAA1 shares 91%, 70%, and 54% sequence identify with mouse SAA2, SAA3, and SAA4, respectively. SAA1 and SAA2 are expressed primarily by the liver and are regulated by the cytokines IL-1, IL-6 and TNF-?, and the levels can increase as much as 1000 fold during inflammation. SAA1 is also associated with certain cancers, atherosclerosis, and rheumatoid arthritis. SAA1 is a precursor of amyloid A, fibril deposits of which may result in inflammatory amyloidosis.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.
Biological function summary
SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.
Pathways
SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system’s reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.
High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.