Abcam, ab216166, Human CT-1 ELISA Kit

Size: 1 x 96Tests
Human CT-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CT-1 with a sensitivity of 3.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 3.2 pg/mL,
Range:23.44 - 1500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CT-1 ELISA Kit (ab216166) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CT-1 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CT-1 with 3.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CT-1 (Cardiotrophin 1) is a secreted member of the IL-6 cytokine superfamily. It is a 201 amino acid protein which binds to and activates the ILST/gp130 heterodimer receptor. CT-1 induces cardiomyocyte hypertrophy
in vitro
and is a biomarker implicated in hypertension. It is highly expressed in heart, skeletal muscle, prostate and ovary. It has lower expression levels in lung, kidney, pancreas, thymus, testis and small intestine. There is little or no expression of CT-1 in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C