Abcam, ab219981, Anti-Mkl1/MRTFA antibody [CL4281]

Size: 100µL
Mouse Monoclonal Mkl1/MRTFA antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MRTFA aa 550-700.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CL4281,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MRTFA aa 550-700. The exact immunogen used to generate this antibody is proprietary information.Q969V6,
Epitope:Binds to an epitope located within the peptide sequence PTLITDSTGTHLVLT as determined by overlapping synthetic peptides.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mkl1 also known as MRTFA is a transcriptional coactivator that plays a role in regulating gene expression. It has a molecular weight of approximately 142 kDa. Mkl1 is widely expressed in various tissues including the heart lung and skeletal muscle. As a coactivator it interacts with serum response factor (SRF) to facilitate the transcription of target genes involved in cytoskeletal dynamics and cell differentiation processes.
Biological function summary
Mkl1 is important in the regulation of actin cytoskeleton remodeling. It does not act alone; it forms part of a dynamic complex with actin monomers. These interactions are essential for the modulation of gene transcription. Mkl1 binds to gene promoters enabling transcriptional activation in response to cellular signals that involve changes in actin polymerization.
Pathways
Mkl1 integrates into the RhoA signaling pathway and the Hippo pathway important for cytoskeleton organization and cell growth control respectively. Within these pathways Mkl1 regulates the activity of SRF coordinating cellular responses to mechanical stress and environmental stimuli. Mkl1 also interacts with other proteins such as myocardin and its family members which are relevant in smooth muscle cell differentiation.
Mutated or dysregulated Mkl1 has been connected to certain leukemias particularly acute megakaryoblastic leukemia. It also relates to cardiac hypertrophy a condition associated with abnormal heart muscle growth. Mkl1 dysregulation leads to altered SRF pathway activity impacting the expression of genes involved in cell growth and differentiation which is critical in these disease processes.