Abcam, ab220162, Anti-YTHDF1 antibody [EPR22349-41]

Size: 100µL / 1mL
Anti-YTHDF1 antibody [EPR22349-41] (ab220162) is a rabbit monoclonal antibody detecting YTHDF1 in Western Blot, IP . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22349-41,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-YTHDF1 antibody [EPR22349-41] (ab220162) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.
What is the molecular weight of YTHDF1?
Anti-YTHDF1 [EPR22349-41] (ab220162) specifically detects a band for YTHDF1 (UniProt: Q9BYJ9) at a molecular weight of 61kDa.
Trusted by the scientific community
Anti-YTHDF1 [EPR22349-41] (ab220162) was first used in a scientific publication in 2019 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-YTHDF1 antibody [EPR22349-41] (ab220162) has been confirmed by Western blot testing in YTHDF1 Knockout mESC cells.
Other related products
We have a range of other formats of antibody clone [EPR22349-41] also available for your convenience: ab220162, Carrier free -
ab253038
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
YTHDF1 also known as YTH domain family protein 1 is a reader protein of N6-methyladenosine (m6A) a common RNA modification. Its mass is approximately 64 kDa. YTHDF1 binds to m6A-modified mRNA and enhances its translation efficiency by recruiting ribosomes. This protein is present in various tissues showing higher expression in brain spleen and lymphoid tissues suggesting a role in development and immunity.
Biological function summary
YTHDF1 affects mRNA stability and translation providing a mechanism for regulating protein synthesis. It operates as part of a complex with other m6A readers like YTHDF2 and YTHDF3 which determine mRNA fate. By promoting translation YTHDF1 impacts cellular responses and adaptation to environmental changes especially during stress and division.
Pathways
YTHDF1 integrates into the mRNA surveillance pathway and translation regulation. It interacts closely with other components such as eukaryotic initiation factors (eIFs) to modulate protein synthesis dynamics. This allows cells to rapidly adjust protein levels in response to cues and maintain homeostasis. Its role is critical in link to ribosomal function and stability of gene expression networks.
YTHDF1 has connections to cancer and neurological disorders. In cancer abnormal YTHDF1 expression associates with tumor progression and metastasis and interacts with proteins like METTL3 involved in m6A deposition. In neurological disorders dysregulation of YTHDF1 influences mRNA translation tied to cognitive functions possibly interacting with neurotransmitter pathways. Understanding these roles shows potential for YTHDF1 as a therapeutic target.