Abcam, ab221198, Anti-Rfa1/Rpa1 antibody

Size: 100µL
Rabbit Polyclonal RPA70 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RFA1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Saccharomyces cerevisiae,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Saccharomyces cerevisiae S288C RFA1. The exact immunogen used to generate this antibody is proprietary information.P22336

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-pH: 6 - 8.5Preservative: 0.05% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rfa1 also known as Rpa1 is a protein component of the Replication Protein A (RPA) complex. It is 70 kDa in size and it plays an important role in DNA metabolism. This protein helps in the stabilization of single-stranded DNA intermediates. Rfa1/Rpa1 expression occurs in various tissues across different species reflecting its importance in cellular processes involving DNA.
Biological function summary
Replication Protein A helps maintain genome stability by providing a platform for the recruitment of other proteins involved in DNA repair and replication. Rpa1 is an important component of the heterotrimeric RPA complex which includes Rpa2 and Rpa3. This complex binds single-stranded DNA during replication and acts as a regulator in both DNA replication and repair pathways preventing DNA from forming secondary structures and protecting it from nucleases.
Pathways
Rfa1/Rpa1 plays a significant role in the DNA damage response pathway and in DNA replication. It works alongside proteins like ATR RAD9 and RAD17 to activate the checkpoint signaling cascade following DNA damage. This ensures proper cell cycle control and DNA repair avoiding cell cycle arrest or mutations. During DNA replication it participates with proteins like DNA polymerases and RFC to ensure accurate DNA synthesis.
Rfa1/Rpa1 is linked to cancer and genomic instability disorders. Its malfunction can lead to an impaired DNA damage response contributing to oncogenesis. The protein's interaction with BRCA1 is significant in breast cancer illustrating how its pathway involvement affects tumor suppression mechanisms. Additionally its relationship with proteins like RAD51 highlights its role in maintaining genome integrity further stressing its importance in preventing pathological conditions.