Abcam, ab221764, Anti-SC5DL antibody

Size: 100µL
Rabbit Polyclonal SC5DL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SC5D aa 50-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SC5D aa 50-100. The exact immunogen used to generate this antibody is proprietary information.O75845

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SC5DL also known as sterol-C5-desaturase or MIG12 is an enzyme important in cholesterol biosynthesis. Weighing approximately 39 kDa SC5DL catalyzes the conversion of lathosterol to 7-dehydrocholesterol by introducing a C5-C6 double bond in the sterol ring. This enzyme predominantly expresses in the liver but is also found in lesser amounts in other tissues such as the brain and skin.
Biological function summary
This enzyme plays a role in the synthesis of cholesterol an essential lipid molecule for membrane structure and function. SC5DL operates within the endoplasmic reticulum often as part of a complex with other enzymes in the cholesterol synthesis pathway. This activity serves not just in producing cholesterol for cell membrane integrity but also generates precursor molecules for steroid hormone production and vitamin D synthesis.
Pathways
SC5DL is an integral part of the mevalonate pathway a critical metabolic pathway for cholesterol synthesis. This pathway involves multiple enzymes and SC5DL works closely with others like HMG-CoA reductase. SC5DL also participates in the Kandutsch-Russell pathway interacting with proteins such as DHCR7 to regulate the flow of sterol intermediates and maintain cholesterol homeostasis.
Alterations in SC5DL activity can impact cholesterol levels and are linked to conditions such as congenital disorders of cholesterol biosynthesis. Mutations or dysfunctions in SC5DL associate with lathosterolosis causing developmental abnormalities due to impaired cholesterol synthesis. Additionally changes in expression of SC5DL and its interaction with proteins like DHCR7 have been studied for their implications in metabolic disorders like Smith-Lemli-Opitz syndrome.