Abcam, ab222875, Human FABP-1 ELISA Kit

Size: 1 x 96Tests
Human FABP-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human FABP-1 in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:>= 0.66 ng/mL,
Range:1.563 - 50 ng/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human FABP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222875) is designed for the quantitative measurement of human FABP-1 protein in plasma, serum, saliva, milk, cerebral spinal fluid and cell culture samples.
The assay employs a quantitative sandwich enzyme immunoassay technique that measures human FABP-1 in approximately 4 hours. A polyclonal antibody specific for human FABP-1 has been pre-coated onto a 96-well microplate with removable strips. FABP-1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human FABP-1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Mitochondrial glutamate oxaloacetate transaminase 2 (FABP-1), also called mitochondrial aspartate aminotransferase (mAspAT) and fatty acid-binding protein, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. GOT is a pyridoxal phosphate-dependent enzyme, which exists in cytoplasmic GOT1 and inner-membrane mitochondrial FABP-1 forms. GOT plays a role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The two enzymes show close homology. FABP-1 is a dimer containing two identical subunits, each 45 kDa with about 401 amino acids. FABP-1 acetylation stimulates the malate–aspartate NADH shuttle activity and oxidative protection. It is also involved in cell proliferation and energy production.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Fatty Acid Binding Protein 1 (FABP-1) also known as Liver-type fatty acid-binding protein (L-FABP) is a small protein with a molecular weight of approximately 14 kDa. FABP-1 is abundantly expressed in the liver and also found in the intestine and kidneys. It functions by binding long-chain fatty acids and other lipophilic substances facilitating their transport within cells. The protein aids intracellular lipid trafficking assisting in the storage and metabolism of fatty acids.
Biological function summary
FABP-1 assists cells in maintaining lipid homeostasis by transporting these fatty acids to different cellular compartments for oxidation signaling or storage. It does not form part of a complex but independently interacts with lipids to buffer their concentration within the cytoplasm. Its role in lipid transport is essential for maintaining metabolic balance and energy production.
Pathways
FABP-1 participates in key metabolic pathways specifically the lipid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways influence energy homeostasis and lipid modulation within cells. FABP-1 interacts with other proteins such as peroxisome proliferator-activated receptor alpha (PPARα) which regulates the expression of genes involved in lipid metabolic processes.
FABP-1 shows connections to liver conditions and metabolic syndrome. Altered levels of FABP-1 can indicate liver injury and could be used as a biomarker for non-alcoholic fatty liver disease (NAFLD). FABP-1 also connects to apolipoprotein E (ApoE) a protein involved in lipid metabolism in relation to the development and progression of atherosclerosis impacting cardiovascular health.