Abcam, ab222910, Anti-LDH-C antibody

Size: 50µL
Rabbit Polyclonal LDHC antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LDHC.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human LDHC.P07864

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LDH-C also known as lactate dehydrogenase C is an enzyme belonging to the LDH family consisting of five similar proteins: LDH-A LDH-B LDH-C LDH-D and LDH-E. LDH-C displays a monomeric mass of about 36 kDa and primarily localizes in the testis. This enzyme catalyzes the reversible conversion of pyruvate to lactate with NADH serving as a cofactor. The kinetic properties and substrate affinities of LDH-C make it distinct from the other LDH isoforms found in various tissues improving its efficiency in the testes.
Biological function summary
Despite LDH-C being expressed much more in spermatozoa than in other tissues it plays a significant role in energy metabolism during spermatogenesis. It acts as part of the tetrameric LDH complex often in combination with other LDH isoforms. This complex facilitates lactate production which sperm cells rely on for energy in low-oxygen environments. The LDH-C form provides metabolic efficiency necessary for sperm motility and function.
Pathways
LDH-C is a critical participant in the glycolytic pathway which plays a central role in energy production under anaerobic conditions. LDH-C's role in converting pyruvate to lactate becomes essential for maintaining the balance of NAD+/NADH an important factor for glycolysis continuity. This enzyme also shares common pathways with LDH-A and LDH-B both important for similar metabolic functions but in other tissues like muscle and liver.
Researchers have noted associations between LDH-C expression levels and male infertility. Abnormal LDH-C forms or its dysfunctional activity may lead to decreased sperm motility or other sperm defects resulting in fertility issues. While no direct connection to other proteins in disease contexts currently known the overlap in function with LDH isoforms particularly LDH-A and LDH-B suggests that altered metabolic environments may influence a range of metabolic disorders.