Abcam, ab223006, Recombinant mouse PKM2 protein (Active)

Size: 20µg
Recombinant mouse PKM2 protein (Active) is a Mouse Full Length protein, in the 1 to 531 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, FuncS, Mass Spec.
Key facts
Purity:>85% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:FuncS, Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity: > 50,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C.,
Accession:P52480,
Animal free:No,
Carrier free:No,
Species:Mouse,
Storage buffer:pH: 8.5Constituents: 30% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target PKM2 also known as pyruvate kinase M2 is a type of enzyme that catalyzes the final step of glycolysis converting phosphoenolpyruvate (PEP) to pyruvate and generating ATP. The PKM2 protein has a molecular weight of about 58 kDa. PKM2 expresses in various tissues including muscles and certain cancer cells where it plays a significant role in metabolic processes. It exists in different isoforms with PKM1 being an alternative form found in pacemaker locations such as the brain and heart.
Biological function summary
PKM2 is important in cellular energy metabolism and regulates the balance between glycolysis and oxidative phosphorylation. This enzyme can exist in both dimeric and tetrameric forms with its activity level depending on its structural state. PKM2 is part of multi-protein complexes that interact with other metabolic enzymes influencing metabolic pathways that impact growth and proliferation in cells.
Pathways
PKM2 is significant in the glycolytic pathway and is also involved in the regulation of the pentose phosphate pathway. It interacts with proteins like HIF-1α a transcription factor that responds to low oxygen levels to modulate metabolic and growth pathways under hypoxic conditions. This interaction affects biosynthetic processes and energy production in rapidly dividing cells such as those in tumors.
PKM2 has a link to cancer and metabolic diseases. PKM2 is often re-expressed in cancer cells where it shifts cellular metabolism to favor rapid growth and proliferation a process known as the Warburg effect. Additionally PKM2 has connections to metabolic disorders such as type 2 diabetes where dysregulated glucose metabolism plays an important role. PKM2’s interaction with proteins like Akt and mTOR can impact signaling pathways associated with cancer progression and metabolic dysregulation.