Abcam, ab223056, Anti-ABCG8 antibody

Size: 50µL
Rabbit Polyclonal ABCG8 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP-binding cassette sub-family G member 8 aa 150-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP-binding cassette sub-family G member 8 aa 150-450. The exact immunogen used to generate this antibody is proprietary information.Q9H221

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ABCG8 also known as ATP-binding cassette sub-family G member 8 is a transporter protein involved in cholesterol homeostasis. It is a part of the ATP-binding cassette (ABC) family with an approximate mass of 74 kDa. ABCG8 is expressed in the liver and small intestine where it plays a role in the export of sterols. This protein functions as a half-transporter and pairs with ABCG5 to form a functional heterodimer which is essential for its function in cellular settings.
Biological function summary
ATP-binding cassette members like ABCG8 participate directly in the movement of lipids across cellular membranes. This transporter is part of a heterodimer complex with ABCG5 that mediates the excretion of sterols into the bile and intestinal lumen. The ABCG5/ABCG8 complex ensures the removal of excess cholesterol and plant sterols from the body maintaining lipid balance and preventing sterol accumulation.
Pathways
The cholesterol transport functions of ABCG8 integrate into the hepatic and intestinal cholesterol efflux pathways. It interacts with proteins and processes in the reverse cholesterol transport pathway and impacts the rate of cholesterol clearance. The ABCG5/ABCG8 heterodimer works alongside proteins like LDL receptors playing a part in a broader network ensuring cholesterol homeostasis and absorption is maintained at optimal levels.
Mutations or malfunctions in ABCG8 link to conditions such as sitosterolemia and gallstone disease. Sitosterolemia is characterized by the accumulation of plant sterols due to impaired function of the transporter complex which affects ABCG5 similarly since they function together. Additionally altered activity of ABCG8 can influence cholesterol crystal formation leading to gallstone development by disrupting the normal biliary sterol composition.