Abcam, ab223750, Anti-MIRO1 antibody [CL1095]

Size: 100µL
Mouse Monoclonal MIRO1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RHOT1 aa 50-150.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CL1095,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RHOT1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.Q8IXI2,
Epitope:Binds to an epitope located within the peptide sequence ERTDKDSRLP as determined by overlapping synthetic peptides.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MIRO1 also known as RhoT1 is a mitochondrial Rho GTPase with a molecular mass of approximately 69 kDa. It functions as a pivotal component in mitochondrial transport and dynamics. This protein localizes to the outer mitochondrial membrane and is highly expressed in various tissues particularly in the brain and muscles. MIRO1 facilitates the anchoring of mitochondria to microtubule motors enabling their movement within the cell which is essential for maintaining proper energy distribution.
Biological function summary
MIRO1 plays a significant role in regulating mitochondrial trafficking along the cytoskeleton. It forms part of a protein complex with TRAK1/2 kinesin and dynein motor proteins. MIRO1's presence in this complex allows it to modulate mitochondrial distribution and dynamics which are essential for neural development and function. It also influences mitochondrial shape and interaction with the endoplasmic reticulum impacting intracellular calcium homeostasis.
Pathways
MIRO1 is an integral component of pathways involving mitochondrial transport and cellular energy homeostasis. It actively participates in the regulation of calcium signaling pathways alongside related proteins like TRAK1. Additionally MIRO1's involvement in the maintenance of mitochondrial dynamics links it to mitophagy pathways. These pathways regulate the removal of damaged mitochondria a vital process for cellular health and longevity.
MIRO1 has connections to neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease. These conditions correlate with impaired mitochondrial trafficking and dynamics. MIRO1 interacts with proteins such as PINK1 and Parkin which contribute to mitochondrial quality control and are involved in the pathogenesis of these diseases. The disruption of MIRO1 function can lead to mitochondrial dysfunction and energy deficits which are underlying factors in these neurodegenerative processes.