Abcam, ab224139, Anti-ATP5J antibody

Size: 100µL
Rabbit Polyclonal ATP5J antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP5PF aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP5PF aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P18859

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP5J also known as ATP synthase subunit F6 is a part of the mitochondrial ATP synthase complex. The ATP5J protein has a mass of about 11 kDa. It is expressed mainly in the inner mitochondrial membrane where it plays an integral role in the process of synthesizing ATP from ADP and inorganic phosphate. ATP5J interacts directly with other subunits of the ATP synthase complex contributing to the functional structure of this vital enzyme complex.
Biological function summary
ATP5J functions in tandem with other components of the ATP synthase complex to facilitate energy production in cells. This complex also known as Complex V catalyzes the final step in oxidative phosphorylation a critical process for cellular energy generation. ATP5J ensures the proper assembly and structural integrity of the ATP synthase complex playing a role in the efficient conversion of the proton motive force into mechanical energy which drives the production of ATP.
Pathways
ATP5J is central to oxidative phosphorylation and ATP synthesis pathways. It interacts closely with key proteins like ATP5A and ATP5B within this pathway. These interactions enable the transduction of electrochemical gradients into usable cellular energy. Furthermore ATP5J's role ensures that ATP synthase maintains its efficiency in energy conversion which is important for meeting cellular metabolic demands.
Mutations or malfunctions of ATP5J can contribute to mitochondrial diseases such as mitochondrial encephalomyopathy. Dysregulation of ATP5J might also relate to disorders like Leigh syndrome where defects in oxidative phosphorylation are common. Other proteins such as COX5A and NDUFS6 also play roles in these conditions highlighting the interconnected nature of mitochondrial function and its impact on human health.