Abcam, ab225569, Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (His tag)

Size: 100µg
Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (His tag) is a Pseudomonas aeruginosa PAO1 Full Length protein, in the 1 to 241 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P54292,
Animal free:No,
Carrier free:No,
Species:Pseudomonas aeruginosa PAO1,
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Regulatory protein RhlR also known as RhlR transcriptional regulator plays an important role in the quorum sensing system of Pseudomonas aeruginosa. It acts mechanically as a transcription factor by binding to specific DNA sequences regulating gene expression linked to virulence factors. RhlR has a molecular mass of around 27 kDa. This protein is expressed within the bacterial cells where it forms part of a communication system that responds to population density.
Biological function summary
The regulatory protein RhlR controls the transcription of genes involved in the production of rhamnolipids pyocyanin and elastase which contribute to bacterial virulence. RhlR forms a part of the complex interplay between LasR and RhlI which comprise the quorum sensing system. This complex communication network allows the bacteria to modulate its pathogenic behavior and adapt to different environments or host conditions.
Pathways
The regulatory protein RhlR serves a primary function within the quorum sensing pathway of Pseudomonas aeruginosa. It interacts significantly with another quorum sensing protein LasR to regulate a hierarchy of genes responsible for pathogenicity. The pathways involving RhlR are critical for the coordinated expression of virulence factors contributing to biofilm formation and increased bacterial resistance to antibiotics.
Regulatory protein RhlR contributes to the pathogenic profile of Pseudomonas aeruginosa in infections like cystic fibrosis and burn wound infections. The enhanced virulence mediated by RhlR impacts the severity and persistence of these infections. RhlR is intricately connected with proteins like LasR through these diseases as they work together in the quorum sensing pathway to promote the bacterium’s survival and adaptation in hostile environments.