Abcam, ab225628, Anti-A-Raf antibody

Size: 100µL
Rabbit Polyclonal A RAF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARAF aa 150-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARAF aa 150-200. The exact immunogen used to generate this antibody is proprietary information.P10398

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab225628 was affinity purified using an epitope specific to A RAF immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The A-Raf protein also called ARAF is a serine/threonine-protein kinase with a molecular mass of about 68 kDa. Scientists frequently refer to it with names like A-Raf or anti A-Raf when discussing its function. A-Raf is expressed in various tissues with higher levels found in the urogenital system and the central nervous system. This protein plays an important role in cellular signal transduction processes influencing cell proliferation and differentiation through phosphorylation activity.
Biological function summary
The A-Raf protein participates in the regulation of cell growth and survival. It forms part of the MAPK/ERK signaling cascade which is essential for transmitting mitogenic signals from the cell surface to the nucleus. A-Raf acts as a modulator within this complex impacting the overall signaling output. The protein is intimately involved in the regulation of the developmental processes influencing cell fate and function.
Pathways
The A-Raf protein is central to the MAPK/ERK pathway a critical signaling pathway involved in the regulation of cellular responses to growth signals. This pathway includes key components like the proteins B-Raf and C-Raf with A-Raf playing specific roles in the modulation and fine-tuning of this signal transmission. It helps mediate signals that control cell division differentiation and secretion ensuring appropriate cellular responses to external stimuli.
Aberrations in A-Raf functions have been linked to cancers particularly urogenital cancers due to its role in uncontrolled cell proliferation. Faulty A-Raf activity can also contribute to neurological disorders because of its expression in the central nervous system and involvement in neuronal signaling pathways. In these contexts it can interact with proteins like B-Raf which are important for maintaining normal cellular signaling and preventing tumorigenesis and neurodegenerative disorders.