Abcam, ab225705, Anti-PUF60/FIR antibody

Size: 100µL
Rabbit Polyclonal PUF60/FIR antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PUF60 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PUF60 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q9UHX1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab225705 was affinity purified using an epitope specific to PUF60/FIR immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PUF60 also known as Far-Upstream Element-Binding Protein 1 (FUBP1) or FIR (FUSE-Binding Protein-Interacting Repressor) is a protein with a molecular mass of about 60 kDa. It expresses in various tissues with significant levels detectable in the nucleus where it functions. This protein plays a role in RNA processing and gene expression regulation by binding to nucleic acids.
Biological function summary
PUF60/FIR is essential for mRNA processing. It is part of the spliceosome complex where it interacts with other splicing factors to facilitate pre-mRNA splicing. PUF60/FIR regulates alternative splicing events which can influence gene expression profiles enabling response to different physiological conditions. It connects with the regulation of cell growth and differentiation through its splicing activities.
Pathways
PUF60/FIR participates in the regulation of transcription and mRNA processing pathways. These pathways include the transcription regulation of MYC a critical protein for cell proliferation and apoptosis. PUF60/FIR modulates the splicing and transcription of RNA polymerase II-dependent genes functioning in interaction with other proteins such as SF3B1 and hnRNP H. This interaction influences the transcriptional activity of gene networks involved in cell cycle progression and survival pathways.
PUF60/FIR has an association with cancer and developmental disorders. Altered expression or mutations in PUF60/FIR link to certain cancers like colorectal cancer due to dysregulation of splicing events. It is also implicated in Verheij syndrome a developmental disorder attributed to haploinsufficiency of PUF60/FIR. In these contexts it interacts with proteins involved in cell cycle regulation and apoptosis like p53 highlighting its broader role in maintaining proper cellular function and development.