Abcam, ab226215, Anti-RNF216 antibody

Size: 100µL
Rabbit Polyclonal RNF216 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RNF216 aa 100-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RNF216 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.Q9NWF9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab226215 was affinity purified using an epitope specific to RNF216 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The molecular structure RNF216 also known as Ring Finger Protein 216 and TRIAD3A is an E3 ubiquitin-protein ligase. RNF216 weighs approximately 101 kDa and contains a RING finger domain which is important for its ligase activity. This protein labels specific target proteins with ubiquitin marking them for degradation by the proteasome. RNF216 can be found in various tissues in the body indicating a widespread expression but it is significantly present in neural tissues.
Biological function summary
RNF216 functions in protein homeostasis and immune system regulation. It acts as a mediator in the disposal of misfolded proteins preventing accumulation that could lead to cellular stress. RNF216 does not work alone; it often interacts with E2 ubiquitin-conjugating enzymes to facilitate substrate targeting and ubiquitination. Furthermore RNF216 is essential for innate immune response modulation through degradation of immune stimulatory proteins.
Pathways
RNF216 takes part in the protein degradation pathway and immune signaling pathways. In the ubiquitin-proteasome system RNF216 collaborates with E2 enzymes as an important player in proteolysis. It also plays a role in regulating the NF-kB pathway by affecting the levels of proteins necessary for NF-kB activation such as TRAF3. These interactions ensure that response to stress and infections is tightly controlled which otherwise could lead to inflammatory conditions.
RNF216 is linked to neurological disorders and immune system dysregulation. Mutations or dysregulation of RNF216 can contribute to Gordon Holmes syndrome which involves movement and endocrine problems due to neural dysfunction. RNF216 also affects immune-related conditions by interacting with proteins like TRAF6 which play a role in immune signaling. Understanding RNF216's role provides insights into potential therapeutic targets for managing such diseases.