Abcam, ab228923, Anti-Aconitase 2 antibody

Size: 100µL
Rabbit Polyclonal Aconitase 2 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACO2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Zebrafish,
Applications:ICC/IF, WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ACO2. The exact immunogen used to generate this antibody is proprietary information.Q99798

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aconitase 2 also known as ACO2 is an enzyme involved in the citric acid cycle which plays an important role in energy production within the cell. This enzyme converts citrate to isocitrate via a cis-aconitate intermediate an essential step in the cycle that generates ATP. Aconitase 2 has a molecular mass of approximately 83 kDa and is primarily expressed in the mitochondria of eukaryotic cells where it functions optimally due to the organelle's environment.
Biological function summary
Aconitase 2 supports cellular energy metabolism by facilitating the conversion of citrate derivatives. It is an iron-sulfur protein integral to both the cytosolic aconitase and mitochondrial varieties that functions as part of the mitochondrial aconitase complex. The enzyme's iron-sulfur cluster gets oxidized under different conditions causing the enzyme to act as a regulatory factor in response to iron levels further linking it with cellular iron metabolism.
Pathways
Aconitase 2 plays a significant role in the tricarboxylic acid (TCA) cycle commonly known as the Krebs cycle and the iron regulatory pathway. In the TCA cycle aconitase 2 acts after citrate synthase and before isocitrate dehydrogenase establishing its role in the middle of the cycle. It is functionally related to iron regulatory proteins particularly IRP1 which can interchangeably bind to iron-responsive elements or possess aconitase activity in its Fe-containing form.
Disruptions in aconitase 2 activity are linked to conditions like Friedreich's ataxia and cancer. Friedreich's ataxia involves mitochondrial dysfunction wherein the decreased activity of aconitase 2 leads to impaired energy metabolism contributing to neurodegeneration. In cancers altered aconitase 2 function can impact cellular proliferation by modifying energy metabolism pathways. Aconitase 2 also interacts with the protein frataxin in the context of these conditions underlining its involvement in disease mechanisms related to mitochondrial dysfunction and iron metabolism.