Abcam, ab230215, Anti-NDUFB5 antibody

Size: 50µL
Rabbit Polyclonal NDUFB5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial aa 100 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O43674

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NDUFB5 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5 is a part of the mitochondrial respiratory chain complex I. It has a molecular mass of approximately 22 kDa. This protein is expressed in tissues with high energy demands such as the brain and muscle. Complex I where NDUFB5 operates plays an important role in cellular respiration initiating the electron transport chain by transferring electrons from NADH to ubiquinone.
Biological function summary
The NDUFB5 protein functions as a subunit within a larger multi-protein enzyme complex called mitochondrial complex I. This complex consisting of 45 subunits is important for oxidative phosphorylation. NDUFB5 is involved in the transfer of electrons facilitating the generation of a proton gradient across the mitochondrial inner membrane. This proton gradient drives ATP production which is essential for energy supply in cells.
Pathways
NDUFB5 participates centrally in the oxidative phosphorylation pathway and is involved in the production of ATP from ADP. The protein's interaction with NADH and ubiquinone links it closely to cellular respiration processes. It collaborates directly with other complex I subunits such as NDUFA and NDUFS to ensure effective energy conversion. Changes in NDUFB5 function can disrupt these pathways affecting overall energy metabolism.
Changes in NDUFB5 activity often relate to mitochondrial disorders particularly those affecting muscle and neurological function such as Leigh syndrome. Mutations affecting its role in complex I can disrupt ATP production. NDUFB5’s dysfunction may connect with other complex I proteins like NDUFS2 leading to bioenergetic failure in cells. These disruptions often result in varied clinical effects highlighting the importance of NDUFB5 in health and disease contexts.