Abcam, ab233468, Peroxynitrite Assay Kit (Cell-based)

Size: 100Test
Peroxynitrite Assay Kit ab233468 uses the fluorescent Peroxynitrite Green dye (Ex/Em 490/530 nm) to assay peroxynitrite. Readout is by fluorometric plate reader or microscope.
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based,
Assay Platform:Microplate reader, Fluorescence microscope

Product details:
Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and understand the role of peroxynitrite (ONOO
) in biological systems. Peroxynitrite Assay Kit (Cell-based) (ab233468) has been developed to address this unmet need. It provides a sensitive tool to monitor ONOO
level in living cells. Peroxynitrite Sensor Green is developed as an excellent fluorescent probe, which can specifically react with intercellular ONOO
to generate a bright green fluorescent product. This kit can be used in fluorescence imaging and fluorescence microplate reader.
Other Notes
Peroxynitrite is a strong oxidizing species and a highly active nitrating agent. Peroxynitrite is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can cause damages to a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Meanwhile, peroxynitrite can also have protective activities
in vivo
by contributing to host-defense responses against invading pathogens. Therefore, peroxynitrite is an essential biological oxidant involved in a board range of physiological and pathological processes.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Peroxynitrite is a reactive nitrogen species with a significant role in cellular signaling. It forms rapidly through the reaction of nitric oxide (NO) and superoxide (O2-) making it a critical part of oxidative stress mechanisms. Peroxynitrite is not a protein but a small molecule so it doesn't have a mass like proteins do. Despite being a molecule it has a significant presence in different cell types especially those under oxidative stress conditions. In interactions it often measures cellular responses to changes in redox balance.
Biological function summary
Peroxynitrite exerts its influence through oxidizing and nitrating various biological macromolecules. It primarily targets proteins lipids and DNA affecting their function and integrity. Although not part of a complex itself it disrupts cellular complexes like mitochondrial electron transport chain components. This prevention of proper ATP production highlights peroxynitrite's role in energy metabolism disruptions.
Pathways
Peroxynitrite plays a role in inflammatory and apoptotic pathways. It interacts with signaling molecules like NF-kB and caspases mediating cellular stress responses. Peroxynitrite modifies tyrosine residues on proteins leading to altered signaling functions. It closely relates to proteins such as SOD (superoxide dismutase) and iNOS (inducible nitric oxide synthase) due to its formation and effects on cellular processes.
Peroxynitrite relates to conditions like neurodegenerative diseases and cardiovascular diseases. Elevated peroxynitrite levels in these diseases indicate oxidative stress and damage to cellular components. During disorders like Alzheimer's disease it interacts with proteins such as amyloid-beta promoting neurotoxic effects. In cardiovascular diseases peroxynitrite interacts with proteins involved in endothelial dysfunction exacerbating disease processes. The presence of antioxidants and scavengers to mitigate peroxynitrite's effects reveal its importance in disease management strategies.