Abcam, ab233726, Recombinant Western Equine Encephalitis Virus E3E2 protein (His tag)

Size: 100µg
Recombinant Western Equine Encephalitis Virus E3E2 protein (His tag) is a Western equine encephalitis virus Full Length protein, expressed in HEK 293T cells, with >80%, suitable for SDS-PAGE, ELISA, WB.
Key facts
Purity:>80% SDS-PAGE,
Expression system:HEK 293T cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P13897,
Animal free:No,
Carrier free:No,
Species:Western equine encephalitis virus,
Storage buffer:Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Western Equine Encephalitis Virus E3E2 is a glycoprotein complex involved in viral entry and fusion. It is alternatively referred to as WEEV E3E2. This protein forms a heterodimer with an estimated molecular mass of approximately 50 kDa. It localizes on the viral membrane surface playing a critical role in mediating the interaction with host cell receptors. The E3E2 complex facilitates viral attachment and subsequent entry into host cells which is essential for the lifecycle of Western Equine Encephalitis Virus.
Biological function summary
The E3E2 complex initiates mechanisms important for infection. It operates as part of the viral envelope embedding within it to mediate fusion between the viral and cellular membranes. This facilitates the release of the viral genome into the host cytoplasm. The E3 segment acts as a regulatory peptide while E2 binds receptors on the host cell surface triggering fusion events. Once the virus enters the host cell it disrupts normal cellular processes to promote viral replication.
Pathways
The E3E2 glycoprotein complex interacts with host cellular processes involved in viral pathogenesis. It is intricately involved in membrane fusion pathways which are central to the viral entry process. The E3E2 complex interacts with host cell proteins such as clathrin and other components of receptor-mediated endocytosis pathways. These interactions facilitate efficient viral uptake and endosomal escape integrated into pathways important to its infectious cycle.
The E3E2 complex has a direct link with Western Equine Encephalitis a mosquito-borne viral disease affecting both equines and humans. This disease can lead to encephalitis causing inflammation of the brain. The E3E2 proteins are involved in the pathogenicity of the virus with their functions critical in leading to disease symptoms. Additionally E3E2 is relevant to studying other encephalitic viruses as it shares functional similarities with proteins from related alphaviruses providing insights into broader viral pathology.