Abcam, ab233743, Anti-1, 3 beta glucan antibody [2G8]

Size: 50µg
Anti-1, 3 beta glucan antibody [2G8] (ab233743) is a mouse monoclonal antibody detecting 1, 3 beta glucan in ELISA . Suitable for Aspergillus nidulans, Candida albicans . - Recombinant format for unrivalled batch-batch consistency
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2G8,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Aspergillus nidulans, Candida albicans,
Applications:ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:ab233743 reacts with 1,3 Β- glucan from Candida albicans, Aspergillus fumigatus and Criptococcus Neoformans and occasionally with Β(1, 6)-linked branching of a single glucosyl residue.ab233743 has been shown to be effective in multiple models of fungal diseases including vaginal and systemic Candida infection, invasive Aspergillosis and Criptococcus Neoformans.

Product details:
What is this antibody validated in?
Anti-1, 3 beta glucan antibody [2G8] (ab233743) is a mouse recombinant monoclonal antibody and is validated for use in ELISA in Aspergillus nidulans, Candida albicans samples.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Produced in cell culture media., Storage buffer-pH: 7.2 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
13-Beta-glucan is a polysaccharide composed of glucose molecules linked by beta-13 glycosidic bonds. This compound is mainly present in the cell walls of fungi bacteria and some plants. It has a complex and varied structure which can impact its molecular weight though specific mass can differ based on source and preparation. 13-Beta-glucan also known as beta-13-glucan is expressed significantly in fungal cell walls serving both structural and protective functions.
Biological function summary
13-beta-glucan plays an important role in the immune recognition process. It forms part of the pathogen-associated molecular patterns (PAMPs) recognized by pattern recognition receptors (PRRs) like Dectin-1 on immune cells therefore triggering innate immune responses. While not part of a larger protein complex 13-beta-glucan interacts closely with immune cells stimulating cytokine production and promoting phagocytosis. This interaction facilitates the body's defense against fungal and bacterial invasions.
Pathways
13-beta-glucan is integral to the immune response and signaling pathways. Its binding to receptors on immune cells like Dectin-1 initiates the NF-kB signaling pathway enhancing the expression of pro-inflammatory cytokines. Similarly because of its engagement in immune processes interactions also include the MAPK pathway. These pathways highlight the collaborative response involving proteins such as Toll-like receptors (TLRs) further coordinating immune activity against pathogens.
13-beta-glucan is especially relevant in the context of invasive fungal infections and sepsis. Elevated levels of this biomarker can indicate a fungal infection guiding diagnostic processes. Moreover its involvement in inflammatory response links it to sepsis where an excessive immune reaction can occur. This reaction involves both the glucan and the TLRs highlighting the interconnected nature of pathogen sensing and immune activation in these conditions.