Abcam, ab234436, Anti-APAF1 antibody [EPR21112-102]

Size: 100µL / 1mL
Knockout Tested Rabbit Recombinant Monoclonal APAF1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 16 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21112-102,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APAF1 also known as apoptotic protease activating factor 1 plays a mechanical role in apoptosis by forming a complex with other proteins to trigger cell death. The APAF1 protein has a molecular weight of approximately 130 kDa. It is expressed in many tissues but occurs highly in the brain and heart. APAF1 interacts with cytochrome c and procaspase-9 to promote the activation of the initiator caspase cascade.
Biological function summary
APAF1 functions as a central component of the apoptosome a multi-protein complex important for programmed cell death. This process is essential for normal development and cellular homeostasis. In the apoptosome APAF1 recruits and activates procaspase-9 leading to the activation of caspase-3. This cascade assures that cells die in a controlled manner preventing damage to surrounding tissues.
Pathways
APAF1 serves an important function in the intrinsic pathway of apoptosis. It mediates cytochrome c release from mitochondria a step essential for apoptosis initiation. Another important pathway involving APAF1 is the apoptotic signaling pathway. Proteins such as Bcl-2 family members and p53 influence APAF1's role in these pathways by regulating cytochrome c release and apoptosome assembly.
APAF1 is notably associated with neurodegenerative diseases and certain cancers. Dysregulation of APAF1 can lead to undesirable apoptosis contributing to neurodegenerative conditions like Parkinson’s disease. Conversely reduced APAF1 activity can result in tumor cell survival impacting cancer progression. Altered expression of APAF1 affects its interaction with proteins like Bcl-2 which can inhibit apoptosis and promote cancer cell growth.