Abcam, ab234761, Anti-ATP8B1 antibody

Size: 100µg / 100µL
Rabbit Polyclonal ATP8B1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP8B1 aa 200-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP8B1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.O43520

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP8B1 also known as FIC1 is a member of the P4-ATPase family responsible for phospholipid flippase activity. It has an approximate mass of 143 kDa. The protein primarily facilitates the transport of phosphatidylserine and phosphatidylethanolamine from the outer to the inner leaflet of the plasma membrane. ATP8B1 is expressed mostly in the liver and small intestine indicating its significant role in those organs.
Biological function summary
ATP8B1 maintains lipid asymmetry in cellular membranes which is critical for cell function and survival. It does not act alone; instead it pairs with CDC50A functioning in a complex to regulate membrane dynamics. This lipid transport activity helps to establish the essential structure of cellular membranes and contributes to bile secretion processes in hepatocytes.
Pathways
ATP8B1 serves an essential function in the bile acid transport pathway. It interacts closely with proteins involved in cholesterol metabolism and excretion such as ABCB11. Efficient bile acid transport is important for maintaining proper liver function and cholesterol homeostasis.
ATP8B1 mutations are directly linked to progressive familial intrahepatic cholestasis type 1 (PFIC1) a severe liver disease resulting in impaired bile flow. Mutations in ATP8B1 also associate with benign recurrent intrahepatic cholestasis (BRIC1). These conditions reveal a connection between ATP8B1 and bile salt export pump (BSEP) highlighting the protein's importance in maintaining liver health.