Thermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays

Thermo Fisher offers an integrated toolkit for cell imaging, automated cell counting, flow cytometry, and targeted functional assays. On this page, you’ll quickly understand the core product lines, pick solutions by application, review representative SKUs, and move straight to purchasing. Iright distributes a broad range of Thermo Fisher products and can quote, source, and support end-to-end.

Core Cell Analysis Lines: EVOS Imaging, Countess Counters, Attune Flow Cytometers

These platforms cover routine culture checks to high-parameter analysis. Start with EVOS for on-bench brightfield/fluorescence imaging; use Countess for rapid counts and viability; escalate to Attune for multiparameter immunophenotyping. Pair instruments with Invitrogen dyes and kits for apoptosis, ROS, calcium flux, and mitochondrial potential.

Core lines at a glance

Quick Selection Guide: Viability, Apoptosis, ROS & Calcium Imaging

Use this fast map when you know the biological question. It pairs common cell-health endpoints with trusted Thermo Fisher tools so you can move from assay concept to a working protocol with minimal friction, whether you read by microscopy, plate, or flow.

• Viability/live–dead (routine culture): Countess 3 with trypan blue for quick counts; upgrade to Countess 3 FL when you need fluorescence-based viability.
  
• Early/late apoptosis: eBioscience Annexin V-FITC Apoptosis Detection Kit (e.g., 88-8005-72/-74) for flow cytometry; multiplex with PI/7-AAD as needed.
  
• Mitochondrial membrane potential (ΔΨm): JC-1 Dye (T3168) for ratiometric green/red readouts by microscopy or flow.
  
• Oxidative stress/ROS: CellROX Green (C10444) for live-cell imaging or flow (fixable after formaldehyde).
  
• Calcium flux: Fluo-4 Calcium Imaging Kit (F10489) for robust Ca²⁺ transients in imaging workflows; Fluo-4 AM also supports flow/plate formats.
  
• Immunophenotyping/multiplex panels: Attune NxT (configurable lasers/channels) or Attune CytPix if you want brightfield images alongside cytometry.

Application Workflows for Reproducible Cell Analysis

The most reliable results come from clear, repeatable steps. Below are reference workflows you can adapt to your lab. Each combines preparation, acquisition, and analysis, minimizing guesswork when switching between imaging, counting, and flow.

Workflow A — Culture QC & viability to apoptosis (flow):

Passage cells → 2) Countess 3 count/viability → 3) Stain with Annexin V-FITC kit (w/ PI) → 4) Acquire on Attune NxT → 5) Export FCS & analyze gates for early/late apoptosis.

Workflow B — Mitochondrial health (imaging):

Culture on coverslips/plates → 2) Stain with JC-1 (T3168) → 3) Image green/red channels on EVOS M5000/M7000 → 4) Calculate red: green ratio for ΔΨm shifts; archive images for longitudinal tracking.

Workflow C — ROS stress (live-cell, imaging or flow):

Treat cells ± stressor → 2) Add CellROX Green (C10444) → 3) Read on EVOS or run on Attune; if needed, formaldehyde-fix to preserve signal and multiplex with antibodies.

Featured Cell Analysis Products & SKUs

Below are representative SKUs to help you move fast. Each item links a well-defined application with a proven instrument or assay so buyers and bench scientists can align quickly before ordering.

Specifications & Resources: Dyes, Kits & Instruments

This section centralizes the practical parameters you check most: excitation/emission maxima and typical readouts. Use it to confirm filter sets and platform compatibility before you book instrument time or place an order; see links for application notes and manuals.

Key assay parameters

FAQs: Cell Imaging, Cell Counting & Flow Cytometry

Before you buy or run a new assay, these are the quick answers most labs need. If your protocol or filter set differs, ask Iright for application support and Thermo Fisher references tailored to your instruments and cells.

1. What’s the difference between Countess 3 and Countess 3 FL?

Both deliver fast counts and viability; the 3 FL adds fluorescence channels for labeled samples (e.g., GFP-positive cells or fluorescent live/dead dyes) while retaining brightfield capabilities.

2. When should I choose Attune NxT vs. Attune CytPix?

Pick Attune NxT for high-throughput, multiparameter flow cytometry with acoustic focusing; choose CytPix when you also want brightfield images during acquisition to validate gates and document morphology. 

3. Can CellROX Green be fixed?

Yes. CellROX Green signal can survive formaldehyde fixation and even limited detergent treatment, enabling post-stain antibody workflows. Avoid alcohol-based fixatives.

4. What filters do I need for JC-1?

Use 488-nm excitation; collect green (~529 nm) for monomers and orange-red (~590 nm) for J-aggregates. Standard FITC and PE channels are commonly used on flow cytometers and widefield microscopes.

Contact Iright for Thermo Fisher Cell Analysis

Tell us what you need—instrument configuration, assay depth, or simply a fast quote—and we’ll match you with the right EVOS, Countess, or Attune setup and the Invitrogen kits to run it. Iright can supply compliant documentation and coordinate delivery to your lab.