Abcam, ab100623, Human PDGF AB ELISA Kit

Size: 1 x 96Tests
Human PDGF AB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PDGF AB in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:10.24 - 2500 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's PDGF AB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AB in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PDGF AB coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDGF AB also known as Platelet-Derived Growth Factor AB is a heterodimeric protein with an approximate mass around 30 kDa. The PDGF AB protein plays an important role in cell proliferation and differentiation by activating its receptor PDGFR. It gets expressed in a variety of cell types including fibroblasts smooth muscle cells and endothelial cells. This growth factor influences cellular behaviors through its interactions with cells in connective tissues where it shows significant levels of expression.
Biological function summary
The PDGF AB protein promotes cellular proliferation and migration particularly in the context of wound healing and development. It forms part of a larger family of PDGF molecules that include homodimers and heterodimers and it requires specific receptor binding to exert its function. As a member of the growth factor family PDGF AB contributes to the signaling pathway that regulates tissue growth and repair processes.
Pathways
PDGF AB fits significantly into the PI3K/AKT and MAPK signaling pathways. These pathways are key in regulating cellular growth and survival and they involve interactions with important proteins like Ras and AKT. The activity of PDGF AB in these pathways affects various downstream molecular processes making it an integral component of cellular signaling networks.
PDGF AB connects to a few pathologies notably atherosclerosis and certain forms of cancer. It often associates with diseases where aberrant cell proliferation and migration occur. For instance in atherosclerosis PDGF AB interacts with proteins such as Integrins and MMPs which contribute to the remodeling of blood vessels. Similarly in oncology the dysregulation of PDGF AB can lead to malignancies with its interaction through growth-promoting pathways.