Abcam, ab100640, Human ICAM1 ELISA Kit (CD54)

Size: 1 x 96Tests
Human ICAM1 ELISA Kit (CD54) is a sandwich ELISA designed to quantify Human ICAM1 (CD54) with a sensitivity of 150 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 150 - 20000 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 150 pg/mL,
Range:150 - 20000 pg/mL,
Assay Platform:Microplate

Product details:
Human ICAM1 ELISA Kit (CD54) ab100640 is a sandwich ELISA to measure Human ICAM1 (CD54) in serum, plasma, cell culture supernatant with a sensitivity of 150 pg/ml.
How the assay works
This assay employs an antibody specific for Human ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human ICAM1 ELISA Kit (CD54) (
ab174445
) from our SimpleStep ELISA
range.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human ICAM1 ELISA Kit (CD54) ab100640 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
Get results in 90 minutes with our SimpleStep ELISA
Human ICAM1 ELISA Kit (CD54) (
ab174445
Optimization may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C