Abcam, ab100660, Human Thyroid Stimulating Hormone ELISA Kit (TSH)

Size: 1 x 96Tests
Human Thyroid Stimulating Hormone ELISA Kit (TSH) is a Sandwich (quantitative) ELISA for the measurement of Human Thyroid Stimulating Hormone (TSH) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 4 pg/mL,
Range:2.744 - 2000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's TSH Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human TSH in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TSH coated on a 96-well plate. Standards and samples are pipetted into the wells and TSH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSH antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TSH also known as thyrotropin or thyroid-stimulating hormone is a glycoprotein hormone with a molecular mass around 28 kDa. It is produced and secreted by the anterior pituitary gland. TSH plays a central role in the endocrine system by stimulating the thyroid gland to release thyroid hormones. These hormones are important for maintaining the body's metabolism growth and development.
Biological function summary
Thyroid-stimulating hormone acts by binding to receptors on the surface of thyroid follicular cells. This binding stimulates synthesis and secretion of thyroxine (T4) and triiodothyronine (T3). TSH is part of the hypothalamic-pituitary-thyroid axis a feedback loop that involves the hypothalamus and pituitary gland to regulate thyroid hormone levels. This axis is vital for maintaining metabolic stability in the body.
Pathways
Thyroid-stimulating hormone plays a significant role in the hypothalamic-pituitary-thyroid (HPT) axis. This pathway regulates the synthesis and release of thyroid hormones involving three main components: thyrotropin-releasing hormone (TRH) from the hypothalamus TSH from the pituitary and thyroid hormones from the thyroid gland. These components work together to ensure proper metabolic homeostasis. Additionally TSH impacts the JAK-STAT signaling pathway influencing cell proliferation and immunity response.
Thyroid-stimulating hormone is closely associated with conditions like Hashimoto's thyroiditis and Graves' disease. In Hashimoto's an autoimmune disorder antibodies attack the thyroid often leading to elevated TSH levels as the body attempts to stimulate the underactive thyroid. In contrast Graves' disease shows reduced TSH levels due to the thyroid being overactive. TSH measurement helps in diagnosing these conditions and understanding the dysregulation of thyroid hormones in these patients.