Abcam, ab104032, Recombinant human SIRT7 protein

Size: 5µg / 10µg
Recombinant human SIRT7 protein is a Human Full Length protein, in the 1 to 400 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:His tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The Specific activity of ab104032 was determined to be 140 RLU/min/ng.,
Accession:Q9NRC8,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIRT7 also known as sirtuin 7 is a member of the sirtuin family of proteins which are class III histone deacetylases. SIRT7 has an approximate mass of 44 kDa and primarily resides in the nucleolus where it is involved in the regulation of ribosomal DNA transcription. It acts by removing acetyl groups from histone H3 at lysine 18 (H3K18Ac) which impacts chromatin structure and gene expression. SIRT7 expression is found in various tissues with higher levels observed in metabolically active tissues such as the liver heart and kidneys.
Biological function summary
SIRT7 plays a significant role in cell proliferation stress resistance and metabolism. It is a component of a nucleolar complex that actively regulates RNA polymerase I-dependent transcription. By deacetylating target substrates SIRT7 enhances rRNA synthesis which is vital for ribosome biogenesis and cellular growth. Additionally SIRT7 influences various cellular processes including mitochondrial homeostasis and DNA damage repair through its deacetylase activity on both histones and non-histone proteins.
Pathways
SIRT7 integrates into the broader framework of cellular regulatory mechanisms. Specifically it is involved in the aging pathway and the p53 signaling pathway. In the aging pathway SIRT7 functions alongside related sirtuins like SIRT1 to modulate lifespan and stress responses. In the p53 signaling pathway SIRT7 interacts with proteins such as p53 and MDM2 influencing apoptosis and cell cycle regulation. These pathways reflect its adaptability in managing cellular survival and growth responses.
SIRT7's involvement is highlighted in cancer and cardiovascular diseases. In cancer its role in promoting ribosomal biogenesis and cell proliferation links it to tumor progression where overexpression correlates with certain cancer types. SIRT7 interacts with oncogenes and tumor suppressor proteins such as MYC and p53 modulating their pathways. In cardiovascular disorders altered SIRT7 activity affects cardiac hypertrophy and myocardial function with connections to proteins that regulate cardiac stress responses such as AMPK and PGC-1α. These associations demonstrate SIRT7's significant impact on disease development and progression.