Abcam, ab104135, Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield

Size: 20mL / 100mL / 250mL / 1000mL
Anti-fade fluorescence mounting medium (ab104135) is a ready-to-use aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. Suitable to prevent rapid photobleaching for IHC, ISH and ICC/IF applications. - Proven performance: cited in over 30 publications - Compatible with our range of fluorescent labeled secondary antibodies - Available in different sizes to fit your experimental needs
Key facts
Form:LiquidSee storage information,
Storage buffer:Preservative: 0.05% Sodium azideConstituents: 84% Water

Product details:
Fluoroshield is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox, phycoerythrin (R-PE), phyocyanin (PC), and allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC.
Mounting media products
For fluorescent cell and tissue staining, Abcam recommends this product (Fluorescence Mounting Medium ab104135), Mounting Medium with DAPI
ab104139
, and Mounting Medium with PI
ab104129
. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI
ab188804
For chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend aqueous Mounting Medium
ab64230
, or organic Limonene Mounting Medium
ab104141
Procedure
Bring the vial to room temperature.
Rinse slide to be mounted with distilled or deionized, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.
Turn the vial upside down and open the dropper to remove any air bubbles.
Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.
Let stand at room temperature for about 5 minutes.
Apply cover slip carefully avoiding air bubbles.
The specimen is ready for visualization under a microscope.
One can seal the edges of cover slip with nail polish, any organic medium or our Organo mounting medium (
ab104141
). If a coverslip is not sealed air bubbles will appear in few days.
For storage it is recommended that the slide be stored in the dark at 2-8°C.
Removal of Coverslip
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deiononized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze, Store in the dark