Abcam, ab104232, Anti-Rad53 antibody

Size: 100µg
Rabbit Polyclonal RAD53 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 84 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Saccharomyces cerevisiae,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rad53 also known as Chk2 is a serine/threonine-protein kinase with a molecular mass of approximately 89 kDa. It plays an essential role in the DNA damage response. It is primarily expressed in the nucleus of eukaryotic cells. Rad53 phosphorylates substrates involved in the cell cycle and DNA repair processes. This protein acts as a signal transducer in the DNA damage checkpoint pathway helping to maintain genomic stability.
Biological function summary
Rad53 regulates the cell cycle by mediating the response to DNA damage. It is part of the conserved cell cycle checkpoint signaling network. This protein interacts with several other checkpoint proteins including Mec1 and Tel1. It modulates the replication fork stabilization and recovery during DNA replication stress. The ability of Rad53 to detect and respond to DNA damage events is key for proper cell cycle progression and the prevention of chromosomal aberrations.
Pathways
Rad53 remains a central component of the DNA damage checkpoint pathway and the replication checkpoint pathway. Rad53 works closely with Mec1 which is an upstream kinase that activates Rad53 in response to DNA damage. The pathway involves other proteins like Dun1 and Sml1 which Rad53 regulates to initiate a proper cellular response. By engaging in these pathways Rad53 contributes to DNA repair and replication fork stability.
Rad53 plays a critical role in cancer and genetic instability disorders. Dysfunctional Rad53 activity can lead to unchecked cell proliferation contributing to the development of cancer. It interacts with proteins such as p53 which is often mutated in cancers. Mutations affecting Rad53 or its associated signaling pathway components can lead to reduced DNA repair capacity increasing susceptibility to genomic instability disorders.