Abcam, ab10579, Anti-TRF2 + TRF1 antibody [TRF-78]

Size: 50µL
Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) is a mouse monoclonal antibody detecting TRF1 in Western Blot, ICC/IF . Suitable for Human . - Over 60 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:TRF-78,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Full Length Protein corresponding to Human TERF1. The exact immunogen used to generate this antibody is proprietary information.P54274

Product details:
What is this antibody validated in?
Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of TRF1?
Anti-TRF2 + TRF1 [TRF-78] (ab10579) specifically detects a band for TRF1 (UniProt: P54274) at a molecular weight of 50kDa.
Trusted by the scientific community
Anti-TRF2 + TRF1 [TRF-78] (ab10579) was first used in a scientific publication in 2004 and has been cited over 60 times in peer-reviewed journals.
Reviewed by scientists
Anti-TRF2 + TRF1 [TRF-78] (ab10579) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRF2 and TRF1 also known as Telomeric Repeat-binding Factors 2 and 1 are proteins essential for telomere maintenance. TRF2 has a molecular mass of approximately 54 kDa while TRF1 is around 56 kDa. These proteins are ubiquitously expressed across various cell types with significant concentrations in proliferative tissues like bone marrow and testis. Both TRF2 and TRF1 are part of the shelterin complex a critical component in regulating telomere length and protecting chromosome ends.
Biological function summary
The shelterin complex which includes TRF2 and TRF1 plays a pivotal role in telomere integrity. They bind to telomeric DNA preventing end-to-end chromosome fusions and inappropriate activation of DNA damage responses. TRF2 and TRF1 are key to preserving telomere structure by facilitating t-loop configuration. This structural maintenance prevents deleterious genomic instability which could otherwise lead to cellular dysfunction or transformation.
Pathways
TRF2 and TRF1 are integral to the telomere signaling pathway. These proteins coordinate with other shelterin components and are essential in managing telomere-associated checkpoint pathways. TRF2 associates closely with proteins like RAP1 aiding in telomere protection and limiting telomerase access. TRF1 interacts with proteins like TIN2 and POT1 further influencing the telomere maintenance pathways by modulating telomeric DNA accessibility and structural organization.
TRF2 and TRF1 are linked to cancer and aging-related conditions. Dysfunction in these proteins disrupts telomere homeostasis potentially leading to tumorigenesis due to unchecked cell division. In cancer abnormal expression of TRF2 connects to proteins such as ATM which play important roles in DNA damage response and repair pathways. Additionally alterations in TRF1 expression can accelerate cellular aging processes highlighting its significance in age-associated diseases.