Abcam, ab106410, Anti-PRDM16 antibody

Size: 100µg
Anti-PRDM16 antibody (ab106410) is a rabbit polyclonal antibody detecting PRDM16 in Western Blot, IHC-P . Suitable for Human, Mouse, Rat . - Over 70 publications
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:WB, ICC, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRDM16. The exact immunogen used to generate this antibody is proprietary information.Q9HAZ2,
Specificity:At least four isoforms of PRDM16 are known to exist; ab106410 will recognize all four.

Product details:
What is this antibody validated in?
Anti-PRDM16 antibody (ab106410) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of PRDM16?
Anti-PRDM16 (ab106410) specifically detects a band for PRDM16 (UniProt: Q9HAZ2) at a molecular weight of 140kDa.
Trusted by the scientific community
Anti-PRDM16 (ab106410) was first used in a scientific publication in 2011 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-PRDM16 (ab106410) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRDM16 also known as the PR-domain containing 16 is a transcription cofactor involved in the regulation of genes. It has a mass of approximately 140 kDa. This protein is found in multiple tissues with high expression levels observed in brown adipose tissue and skeletal muscle. PRDM16 regulates gene expression by interacting with other transcription factors and modifying chromatin structure.
Biological function summary
PRDM16 is essential in the determination of brown adipose tissue and skeletal muscle differentiation. It belongs to a complex that includes C/EBPΒ and PPARγ which are pivotal for the conversion of myoblasts into thermogenic brown fat cells. This activity contributes to the generation of cells specialized for heat production which aids in maintaining body temperature.
Pathways
PRDM16 functions are linked to cellular metabolism and thermogenesis. It plays a substantial role in the Wnt/Β-catenin signaling pathway where it influences energy balance. In this pathway PRDM16 interacts with Wnt signaling regulators and coactivators that control adipocyte differentiation and energy expenditure.
PRDM16 plays a role in myoblastic leukemia and obesity-related conditions. Changes in the function or expression of PRDM16 can contribute to pathological transformation leading to leukemia with proteins C/EBPΒ and PPARγ also involved in related mechanisms. In obesity altered PRDM16 activity affects the formation and function of brown adipose tissue potentially disrupting normal energy metabolism and storage.