Abcam, ab106464, Anti-LXR alpha antibody

Size: 100µg
Rabbit Polyclonal LXR alpha antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human NR1H3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NR1H3. The exact immunogen used to generate this antibody is proprietary information.Q13133,
Specificity:At leastthree isoforms of LXR alpha are known to exist; ab106464 will recognize all three. ab106464 is predicted to not crossreact with LXR beta.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab106464 was purified by affinity chromatography via a peptide column., Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LXR alpha also known as NR1H3 is a nuclear receptor protein involved in the regulation of lipid metabolism and inflammation. It has a molecular mass of approximately 55 kDa. Expressed mainly in the liver adipose tissue macrophages and intestines LXR alpha acts as a transcription factor controlling the expression of genes important for cholesterol homeostasis. It binds to DNA at specific LXR response elements usually forming heterodimers with the retinoid X receptor (RXR).
Biological function summary
LXR alpha plays a critical role in lipid metabolism and cholesterol efflux. It is part of a larger LXR and CO complex which contributes to the regulation of genes that manage cholesterol absorption transport and excretion. For instance LXR activation increases the expression of ATP-binding cassette transporters such as ABCA1 and ABCG1 which are essential for transporting cholesterol out of cells and facilitating reverse cholesterol transport.
Pathways
The function of LXR alpha is significant in both the cholesterol catabolism pathway and the inflammatory response pathway. Within these pathways it upregulates genes involved in cholesterol catabolism providing a mechanism to prevent excess accumulation in cells and tissues. LXR alpha interacts closely with other nuclear receptors and proteins like PPARs (peroxisome proliferator-activated receptors) highlighting its role in lipid signaling networks.
LXR alpha is linked to atherosclerosis and Alzheimer’s disease. Its ability to regulate cholesterol transport and immune function demonstrates involvement in the pathogenesis of atherosclerosis by influencing macrophage-derived foam cell formation. Additionally improper cholesterol metabolism is associated with Alzheimer’s disease where LXR alpha's interaction with amyloid precursor protein (APP) may influence disease progression connecting cholesterol dysfunction with neurodegenerative processes.