Abcam, ab107159, Anti-Iba1 antibody - Microglia marker

Size: 100µg
Anti-Iba1 antibody (ab107159) is a goat polyclonal antibody detecting Iba1 in IHC-P . Suitable for Rat . - Over 150 publications
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:If looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846. This has the same immunogen as ab5076. In recent testing in IHC-P this antibody only worked in Rat tissue, therefore we have removed Human and mouse as a "does not react with" species. However, feedback from our customers and publications suggest that this antibody recognizes human and mouse. Nevertheless, we cannot guarantee this antibody in these species.

Product details:
What is this antibody validated in?
Anti-Iba1 antibody (ab107159) is a goat polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Rat samples.
Trusted by the scientific community
Anti-Iba1 (ab107159) was first used in a scientific publication in 2011 and has been cited over 150 times in peer-reviewed journals.
Reviewed by scientists
Anti-Iba1 (ab107159) has over 15 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 3% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.
Biological function summary
Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.
Pathways
Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.
Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.