Abcam, ab108819, Rat Ceruloplasmin ELISA Kit (CERP)

Size: 1 x 96Tests
Rat Ceruloplasmin ELISA Kit (CERP) is a Sandwich (quantitative) ELISA for the measurement of Rat Ceruloplasmin (CERP) in Rat in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Cell culture supernatant, Serum, Cell Lysate,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 74 ng/mL,
Range:1 - 10 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Abcam's Ceruloplasmin (CERP) rat
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in urine and cell culture supernatants.
A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols