Abcam, ab108891, Human PAI1 ELISA Kit (SERPINE1)

Size: 1 x 96Tests
Human PAI1 ELISA Kit (SERPINE1) is a Sandwich (quantitative) ELISA for the measurement of Human PAI1 (SERPINE1) in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum, Tissue,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 19 pg/mL,
Range:0.15 - 1.25 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Abcam's PAI1 (SERPINE1) Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum, tissue extracts and cell culture supernatants.
A PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.
This assay recognizes both natural and recombinant Human PAI-1.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols