Abcam, ab109862, Anti-Complex III Immunocapture antibody [11A51H12]

Size: 100µg
Mouse Monoclonal UQCRFS1/RISP antibody. Suitable for IP and reacts with Human, Cow samples. Cited in 10 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:11A51H12,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Cow, Human,
Applications:IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:100 µg ab109862 can capture at least 10 µg Complex III from 1 mg solubilized bovine heart mitochondria.

Product details:
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Properties and Storage Information:
Form-Liquid, Purification notes-ab10862 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complex III also known as cytochrome bc1 complex or ubiquinol-cytochrome c reductase is an important component of the mitochondrial electron transport chain. This protein complex has a mass of approximately 500 kDa and is located in the inner mitochondrial membrane. Complex III plays a mechanical role by facilitating the transfer of electrons from ubiquinol to cytochrome c while simultaneously pumping protons across the mitochondrial membrane. This action helps in establishing the electrochemical gradient used for ATP synthesis.
Biological function summary
The molecular actions of Complex III are integral to aerobic respiration. It forms part of the larger mitochondrial respiratory chain complex working closely with Complexes I II and IV. Complex III through its electron transport and proton translocation activities helps in the production of ATP an energy currency vital for cellular processes. It also influences the generation of reactive oxygen species (ROS) which can serve as cellular signals or cause damage under certain conditions.
Pathways
Complex III is embedded in the oxidative phosphorylation pathway as a central component. It interacts with other protein complexes like Complex I and IV which are also important for the production of ATP. By transferring electrons it plays a role in maintaining the efficiency of the electron transport chain. Even slight dysfunctions in Complex III's activities can lead to disruptions in the oxidative phosphorylation pathway affecting overall cellular energy metabolism.
Deficiencies or mutations in Complex III correlate with mitochondrial diseases and neurodegenerative disorders. For example Complex III dysfunction has been connected with diseases such as mitochondrial myopathy and Parkinson's disease. In such conditions the protein's activity directly impacts ATP production and ROS levels linking it to disruptions in cellular homeostasis. Mutations in its associated proteins like cytochrome c can exacerbate these disorders highlighting Complex III's significance in maintaining physiological function.