Abcam, ab110168, Mitochondria Isolation Kit for Tissue

Size: 1Kit
Mitochondria Isolation Kit for Tissue (ab110168) is designed for efficient isolation of mitochondria from mammalian tissue using differential centrifugation. Suitable for Western blot, immunocapture or activity assays. - Provides reagents for up to 10 isolations - Proven performance: cited in over 65 publications
Key facts
Assay time:1h,
Sample types:Tissue

Product details:
ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze
Tested tissues: brain, liver and heart

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C