Abcam, ab11309, Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker

Size: 50µL
Mouse Monoclonal TBB2 antibody - conjugated to Cy3®. Suitable for ICC/IF and reacts with Chicken samples. Cited in 12 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Tubb5.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:TUB 2.1,
Isotype:IgG1,
Conjugation:Cy3®,
Excitation/Emission:Ex: 552nm, Em: 565nm,
Carrier free:No,
Reacts with:Chicken,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Full Length Protein corresponding to Rat Tubb5. The exact immunogen used to generate this antibody is proprietary information.P69897,
Epitope:Ab11309 specifically recognizes an epitope in the carboxy-terminal part of beta-tubulin (between amino acids 281-446).

Product details:
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The product is prepared by conjugation of Cy3 to purified monoclonal anti-beta-tubulin antibody. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore. F/P Molar Ratio: (Cy3:Ab) 3 to 9.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.
Biological function summary
This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.
Pathways
Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.
Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.